Description
Intended Uses: Influenza A IgG Anlibody ELISA Test Kit has been designed for the deteclion and quantitative determination of specific IgG antibodies against Influenza A in serum and plasma. For in vitro diagnostic use only.
Principle of the Assay: Influenza A IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Influenza A antigen is bound on the surface of the microtiter strips. Diluted serum or ready-to-use standards are pipetted into the wells of the microliter plate. A binding between the IgG antibodies of the serum and the Immobilized Influenza A antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use antihuman-lgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step. the substrate (TMB) solution is pi petted and incubated for 20 minutes. inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color